Abstract:

Objective   To observe effects of omega-3 polyunsaturated fatty acids (PUFAs) on keratinocyte proliferation and fibroblast differentiation. Methods   Keratinocytes and fibroblasts were cultured in vitro. The cells were treated with omega-3 PUFAs at different concentrations. BrdU assay was used to examine the keratinocyte proliferation. The cell toxicity of eicosapentaenoic acid (EPA) was assessed by MTT. ELISA assay was used to observe the expressions of ɑ-SMA and human collagen-1. Results   At a concentration of 200μmol/L, EPA significantly promoted keratinocyte proliferation, increased the expression of ɑ-SMA and decreased the expression of human collagen-1 in fibroblasts. Conclusion   EPA could increase keratinocyte proliferation and promote fibroblast differentiation, which might promote wound healing.

Key words: Omega-3 polyunsaturated fatty acids; Keratinocytes; Fibroblasts; Wound healing; Proliferation; Differentiation