Abstract:

Objective   To investigate the effect and mechanism of adenovirus-mediated BMP2 (Ad-BMP2) gene transfection to Tca8113 cells in vitro, and observe the effect of BMP2 gene transfection on the severe combined immunodeficiency (SCID) mice with tongue cancer line Tca8113 in vivo. Methods   Tongue cancer cell line Tca8113 was cultivated in vitro and transfected with different densities of Ad-BMP2. Expressions of BMP2, Smad1 and Smad5 in the Ad-BMP2 and PBS groups were detected through Western blot. Tumor models in SCID mice inguinal subcutaneous were established and injected with Ad-BMP2 gene. The changes of tumor volume and transferring circumstances were observed. Results   The result demonstrated that the expression levels of Smad1 and Smad5 were positively correlated to the BMP2 transfection density(P＜0.05). After the Ad-BMP2 gene was transfected into Tca8113 cells, the tumors diameter of  the SCID mice grew about 0.6cm in 2 weeks, and the tumor growth was obviously inhibited. The metastases of tumor in the liver, spleen, kidney and tomentum majus of the SCID mice were not detected. Conclusion   Ad-BMP2gene is successfully transfected to Tca8113 cells and can significantly increase the expressions of Smad1 and Smad5 proteins. BMP2 can significantly inhibit the growth of Tca8113 tumor grafted on the SCID mice.  

Key words: Gene transfection； Tongue cancer cells； Bone morphogenetic proteins2；  Severe combined immunodeficiency mice； Transplanted tumor