JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2012, Vol. 50 ›› Issue (9): 127-.

• Articles • Previous Articles    

Sequence analysis for 5′untranslated region of
genomes from enterovirus 71

YUAN Xiao-jing1,  WEN Hong-ling1, GAO Feng2, XU Hong-Zhi1, SI Lu-ying1,
WANG Dong-xu1, SONG Yan-yan1, ZHAO Li1, WANG Zhi-yu1   

  1. 1. Department of Virology, School of Public Health， Key Laboratory for Experimental Teratology of the Ministry of
     Education, Shandong University, Jinan 250012, China；
    2. Department of Infectious Diseases, Linyi People′s Hospital, Linyi 276000, Shandong, China
  • Received:2012-04-25 Online:2012-09-10 Published:2012-09-10

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Abstract:

Objective   To investigate the sequence of  5′ untranslated region (5′ UTR) in genomes from enterovirus 71 strains isolated in Linyi, Shandong province in 2009 and analyse the candidate locuses that were related to neurovirulence. Methods   8 EV71 strains were isolated from the patients′ feces samples.  5′UTR in genomes were amplified by RT-PCR and the sequences were analyzed by DNAstar and MEGA 4 softwares. Results   Lengths of 5′UTR for the 8 strains were in the range of 741-745 nt. Nucleotide sequence identities for 5′UTR were 97.0%-99.9%. The 5′UTR nucleotide sequences of 8 EV71 strains exhibited highest homology to the subtype C4 of EV71. Two mutations (G265A and G703T) in 5′UTR were found by nucleotide sequence analysis. The phylogenetic analysis based on 5′UTR showed that 8 EV71 strains had the closest genetic relationship with the representative strains of C4 subtype of EV71. Conclusion   The 8 EV71 strains belong to C4 subtype. Nucleotide mutations (G265A and G703T) might be relevant to neurovirulence of EV71.

Key words: Hand, foot and mouth disease; Enterovirus 71; 5′untranslated region; Phylogenetic analysis； Neurovirulence

CLC Number: 

  • R373.2
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[2] WANG Dong-xu1, WEN Hong-ling1, DONG Hua1, SUN Cheng-xi1, SI Lu-ying1,YUAN Xiao-jing1, CHU Fu-lu1, ZHAO Li1, WANG Zhi-yu1,2. Immunological effect of different immunization schedules on enterovirus 71 [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(3): 129-.
[3] LI Zhi-hui, YUE Ying-ying, SONG Nan-nan, LI Peng, MENG Hong. Toward constructing a mucosa vaccine for enterovirus 71 [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(2): 25-.
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[5] LI Peng1, ZHANG Ying2, YUE Ying-ying1, SONG Nan-nan1, LI Zhi-hui1, GAI Zhong-tao2, MENG Hong1. Complete genome sequence analysis of enterovirus 71 JN200804 strain isolated in Shandong Province [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(10): 113-117.
[6] LI Zhi-hui， LI Peng， SONG Nan-nan， YUE Ying-ying， JI Xuan， ZHAO Yuan-hao， MENG Hong. Application of sensitized sheep erythrocytes in cell tropism of EV71 [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(11): 33-.
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