JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2012, Vol. 50 ›› Issue (8): 46-50.

• Articles • Previous Articles     Next Articles

Differential  expressions of HSP60 in docetaxel treatment on
advanced hormone-resistant prostate cancer

WANG Hai-bin, MA Wei-ming, XIAO Pan, MA Tian-jia, ZHANG Huai-qiang   

  1. Department of Urology, The Second Hospital of Shandong University, Jinan 250033, China
  • Received:2012-02-29 Online:2012-08-10 Published:2012-08-10

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Abstract:

Objective   To identify the different expressions of heat shock protein 60(HSP60) in apoptosis and drug resistance induced by docetaxel in androgen independent prostate cancer PC-3 cells, and investigate its significance. Methods   Apoptotic PC-3 cells were induced by 20μmol/L docetaxel. Docetaxel-resistant PC-3 cells were produced by breeding with increasing doses of docetaxel. Fluorescence two-dimensional difference gel electrophoresis(DIGE) was used to quantatively screen the differentially expressed proteins between untreated PC-3 cells and apoptotic PC-3 cells, docetaxel-sensitive PC-3 cells and docetaxel-resistant PC-3 cells. The constituents were analyzed by matrix-assisted laser desorption／ionizafion time of flight mass spectrometry(MALDI-TOF-MS) and the expression of HSP60 was detected. Results   The expression of HSP60 was up-regulated in docetaxel-induced apoptotic PC-3 cells, and down-regulated in docetaxelresistant PC-3 cells. Conclusion   The expression level of HSP60 is high in the apoptotic PC-3 cells and low in the docetaxel-resistant PC-3 cells. It suggests that HSP60 might play an important role in apoptosis and docetaxelresistance of PC-3 cells.

Key words: Docetaxel; Heat shock protein 60; Apoptosis; Drugresistance; Proteomics

CLC Number: 

  • R737.25
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