Abstract:

Objective   To investigate the anti-proliferation activity of luteolin in lung cancer A549 cells and to explore the effects of luteolin combined with chemotherapy. Methods   Cell cycle and apoptosis of A549 cells were detected by flow cytometry with propidium iodide (PI) staining and Annexin-V. FITC and PI dual parameter markers to clarify the mechanism of luteolin on inhibiting cell proliferation. The inhibition effect of luteolin on the cell migration was detected by the mitochondrial membrane potential test and the scratch test. The effect on actin was researched through the confocal microscope. Chemotherapy effect of luteolin with cisplatin was detected by the cell real-time detection system. Results   Luteolin could apparently inhibit cell proliferation and migration compared with the control group. Luteolin could enhance the anti-tumor effect of chemotherapy. Conclusion   Luteolin can block the cell cycle in G1 phase, induce cell apoptosis, and inhibit cell migration. Luteolin enhances anti-tumor effect of chemotherapy.