Abstract:

Objective   To observe the impacts of procyanidins(PC) on the proliferation of hepatic stellate cell(HSC) and production of tissue inhibitor of matrix metalloprotease-1(TIMP-1) induced by leptin. Methods   Sulforhodamine B assay was performed to test the proliferation of HSC. The probe of reactive oxygen species (ROS) 2, 7-dichlorfluorescein-diacetate was used to detect the production of ROS. Enzyme-linked immunosorbent assay was used to detect the concentration of TIMP-1. Results   PC at concentrations of 50， 100， 200μg/mL could inhibit the proliferation of HSC induced by leptin (P<0.05). Meanwhile, it had the inhibitory effect on the production of ROS (P<0.05). The PC groups above could also inhibit the generation of TIMP-1(P<0.05). There were statistically significant differences among the groups (P<0.05). Conclusion   PC might inhibit the HSC proliferation and TIMP-1 production through inhibiting the oxidative stress induced by leptin.