Abstract:

Objective   To precisely trace and separate mesocortical dopaminergic (DA) neurons and detect the effects of paliperidone on their action potentials. Methods   According to stereotaxic apparatus, fluorescent retrograde tracer was injected to the entorhinal cortex of rats′ brains. The brains were removed after being perfused to be made into frozen sections. Immunohistochemistry was adopted for tyrosine hydroxylase (TH) staining. The nerve cells labeled by the tracer were sorted by the fluorescence intensity, and cultured. The generations and changes of action potentials before and after antipsychotic paliperidone treatment were detected by using cell patch-clamp technique for the current injection. Results   The midbrain DA neurons which projected to the cerebral cortex were labeled accurately, showing TH-positive, and located in the ventral tegmental area. DA neurons which were marked and filtered out showed a typital action potential after being induced by electrical stimulation. After antipsychotic paliperidone treatment, the amplitude of action potential appeared lower(P<0.05), and the width became smaller(P<0.01). Conlusion   The mesocortical DA neurons which project to the frontal brain can be separate accurately. Paliperidone can reduce the amplitude and width of the action potentials of the cells, thus reduce the excitability of the cells.

Key words: Type 2 diabetes mellitus; Screening; Fasting capillary blood glucose; Fasting plasma glucose； 2-hour plasma glucose； Glycosylated haemoglobin； Diabetes risk score; Receiver operating characteristic, Paliperidone; Dopamine; Neurons; Stereotaxic techniques; Action potentiais