Abstract:

Objective   To investigate the effects of oxidative stress on expressions of NF-κB P65, MMPs/TIMPs in porcine trabecular meshwork cells(PTCs) and to explore the role of the NF-κB signaling pathway in trabecular extracelluar matrix(ECM) remodeling. Methods   The PTCs were cultured and passaged. The 3rd generation trabecular cells were cultured without serum and stimulated by 1mmol/L H2O2 with or without pretreatment of pyrrolidine dithiocarbamate(PDTC). Expressions of NF-κB P65, MMP-2, MMP-3 and TIMP-1 were analyzed with immunocytochemistry and RT-PCR.  The data were statistically analyzed. Results   Expressions of NF-κB P65, MMP-2, MMP-3 and TIMP-1 were significantly increased by H2O2  in PTCs. PDTC partly reversed H2O2 induced expressions of MMP-2 and MMP-3. Expression of TIMP-1 had no significant variation after pre-treatment of PDTC. Conclusion   Oxidative stress can induce expression of NF-κB P65 in PTCs. The NF-κB signaling pathway was involved in trabecular ECM remodeling. Other signaling pathways may be related to the process.

Key words: Oxidative stress; Trabecular meshwork cell; NF-κB signaling pathway; Pyrrolidine dithiocarbamate; Extracelluar matrix; Remodelling