Abstract:

Objective   To investigate the anti-inflammatory effect of burdock fructo-oligsaccharide (BFOS) on the inflammatory model of RAW264.7 cells. Methods   The inflammation model was constructed by treating RAW264.7 cells with lipopolysaccharide (LPS), and treated with different doses of BFOS. Expressions of Cyclooxygenase (COX-2), Nitric oxide synthases (iNOS), monocyte chemotactic protein-1 (MCP-1) and Interleukin-6(IL-6) were detected by realtime PCR. Expressions of MCP-1 and IL-6 were determined by ELISA before and after the treatment with BFOS. Expressions of COX-2, iNOS, phosphorylated IκB, NF-κBp65, extracellular signal-regulated (Erk), P38 and c-Jun Nterminal kinase (JNK) were detected by Western blot. Results   The expression levels of iNOS，COX-2，MCP-1 and IL-6 of RAW264.7 cells stimulated by LPS increased, and BFOS significantly decreased them . BFOS decreased the expressions of p-IκB，p-NF-κBp65，p-Erk，p-P38，and p-JNK compared to the control. Conclusion   BFOS may exert anti-inflammatory effect on RAW264.7 cells stimulated by LPS.

Key words: Burdock fructo- oligsaccharide; Anti-inflammatory effect; RAW264.7 cell line; Nuclear factor-κappa B； Mitogen-activated protein kinase