JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2011, Vol. 49 ›› Issue (9): 132-.

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Clinical staging in non-small cell lung cancer by surface-enhanced laser desorption/ionization-time of flight-mass spectrometry

XU Ji-ying, WANG Dong-fang, WANG Ming-ming, JIANG Man, BI Yu-li, HAN Ming-yong   

  1. Cancer Center, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China
  • Received:2010-10-27 Online:2011-09-10 Published:2011-09-10

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Abstract:

 Objective   To detect proteomic changes in non-small cell lung cancer and find the association of serum protein profiles with the clinical stage. Methods   Twenty-eight cases of stage Ⅰ-Ⅱ non-small cell lung cancer and 41 cases of stage Ⅲ-Ⅳ  were detected. Protein fingerprints were detected by surface-enhanced laser desorption/ionizationtime of flight-mass spectrometry(SELDI-TOF-MS) and a CM10 chip. Bioinformatics was used to analyze the relationship between the serum proteomic fingerprint and clinical staging in non-small cell lung cancer. Results   Comparing proteomic changes between stage Ⅰ-Ⅱ and stage Ⅲ-Ⅳ groups, 68 discrepant proteins were selected. The best combination consisted of 5632m/z and 13779m/z, which were highly expressed in stage Ⅲ-Ⅳ non-small cell lung cancer and lowly expressed in stage Ⅰ-Ⅱ, with an accuracy of 0.587 and a compatibility of 79.5%， Kappa=0.32. Conclusions   SELDI-TOF-MS can be used to detect serum protein profiles in non-small cell lung cancer, and selected 5632m/z and 13779m/z might be markers for clinical staging before operation. 

Key words: Lung carcinoma； Medical informatics； Clinical diagnosis； Proteomic profile

CLC Number: 

  • R734.2
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