Abstract:

Objective      To investigate the effect of Xingnaojing(xnjj) on the apoptosis of hippocampal neurons in rats with subarachnoid hemorrhage(SAH).  Methods     The SAH model was established by two time autologous blood injection into the cisterna magna of adult male Wistar rats. 35 rats were randomly divided into 5 groups naive, saline control, SAH, xnjj treatment 1, and xnjj treatment 2 groups. Gene expressions of Caspase-3, Bax, and Bcl-2 in different groups after SAH were detected with Real-Time PCR. Further, Western blot was applied to determine protein changes of these three molecules after SAH. Results     Compared with the control group, Caspase-3, Bax, and Bcl-2 increased in varying degrees in the hippocampus of the SAH group(P＜0.05), while in Xingnaojing treatment groups, Caspase-3 and Bax, but not Bcl-2, decreased when compared with the SAH group(P＜0.01). Conclusion      Xingnaojing shows an obvious neuroprotective effect on hippocampal neurons by inhibiting SAHrelated apoptosis.

Key words: Subarachnoid hemorrhage； Models, animal； Hippocampal; Apoptosis； Rats, Wistar