JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2011, Vol. 49 ›› Issue (5): 6-.

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Bone marrow mesenchymal stem cells protect INS-1 cells from  hydrogen peroxide-induced apoptosis

SUN Hui-huan1, LIU Fu-qiang1, GONG Lei1, XIAO Fang1, HOU Xin-guo1,  SUN Yu1, SONG Jun1, CHEN Li1, WANG Xu-ping2   

  1. Qilu Hospital of Shandong University 1. Department of Endocrinology, 2. Key Laboratory of Cardiovascular Remodeling and
    Function Research, Chinese Ministry of Education and Chinese Ministry of Health, Jinan 250012,  China
  • Received:2011-01-06 Online:2011-05-10 Published:2011-05-10

Abstract:

Objective     To investigate the effect of bone marrow mesenchymal stem cells (BMSCs) on hydrogen peroxide (H2O2)-induced apoptosis in INS-1 cells.  Methods     The apoptotic INS-1 cells, induced by H2O2, were co-cultured with BMSCs. The experiment consisted of 4 groups: group A(control group), INS-1 cells only; group B, treated with H2O2 ; group C, coculture of INS1 cells and BMSCs; and  group D, co-culture with H2O2 treatment. Cell viability was measured by MTT. Annexin-V/PI doubled staining of flow cytometry was used to quantify the apoptosis ratio. Results     INS-1 cell viability was reduced by H2O2 in a dosedependent manner. With Transwell co-culture, H2O2 induced INS-1 cell apoptosis ratio was significantly lower than that of H2O2 group(P<0.05).  Conclusion     BMSCs can protect INS-1 cells from apoptosis induced by H2O2 and may have therapeutic potentials to prevent and treat diabetes.

Key words: INS-1; Apoptosis; Bone marrow mesenchymal stem cells; Co-culture

CLC Number: 

  • R587.1
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