Abstract:

Objective    To study the effects of high-concentration distilled water(DW) and cisplatin administered locally in a short time on osteosarcoma (OS) cells. Methods    Cultured osteosarcoma cells, U2-OS, were divided into the DW group, the DW31.25 group, the DW62.5 group, the DW125 group, the DW250 group and the DW500 group according to the corresponding concentrations of cisplatin applied, i.e. 31.25μg/mL, 62.5μg/mL, 125μg/mL, 250μg/mL and 500μg/mL, respectively. Then, osteosarcoma cells were processed for 5 minutes. At 0, 24, 48, 72 and 96h, the inhibition rates of tumor cells were determined by MTT assay. Changes of cells in morphology and quantity were observed under inverted microscopy. Results    Among the groups, there was no significant difference in the inhibition rates at 0h. Though no differences were observed among the DW62.5 group, the DW125 group, the DW250 group and the DW500 group at 96h, the inhibition rates were significantly higher than those of the DW group and the DW31.25 group. In the former 4 groups, hardly any osteosarcoma cells survived at 96h while massive osteosarcoma cells survived in the DW group. Conclusion    Highconcentration distilled water and cisplatin administered in a local and short-time manner produces strong killing effect on osteosarcoma cells.

Key words: Osteosarcoma; Cisplatin; Distilled water