Abstract:

Objective    To explore the role of Src kinase in the induction of spinal long-term potentiation (LTP) induced by the D1/D5 receptor agonist. Methods    C-fiber evoked field potentials were recorded at the superficial layer of the spinal dorsal horn at the lumbar enlargement by extracellular recording technique.  Results    ① SKF38393 (SKF, 250μmol/L)-induced LTP of C-fiber evoked field potentials was blocked by SCH 23390 (SCH, a D1/D5 receptor antagonist, 20μmol/L).  ② Genistein (200μmol/L), a selective Src kinase inhibitor, blocked the induction of spinal LTP induced by SKF.  ③ NR2B antagonist Ro256981 (Ro, 50μmol/L) prevented spinal LTP by SKF.  Conclusion    Activation of Src kinase and NR2B in the spinal dorsal horn may be crucial for the induction of spinal LTP by D1/D5 receptor agonists.

Key words: Src-Family kinases; Receptors， N-methyl-D-aspartate； Long-term potentiation; Spinal cord; Receptors， dopamine