Abstract:

 Objective     To detect small RNAs and identify those differentially expressed between sham operation (F group) and cerebral ischemia reperfusion (B group) in rats. Methods     Total RNA was extracted from the hippocampus tissue from the two groups. Solexa high-throughput sequencing was used to obtain miRNA expression profiles and bioinformatics analyses were performed. Results     6833331 and 6407399 reads were obtained in the F and B groups, respectively, with a average length of 22 nucle-otides in either group. All reads were examined against the miRBase15.0, Genbank and Rfam (9.1) databases, and were annotated into different categories. For groups F and B, MiRNA sequences account for 76.77% and 75.22%, respectively, of the respective total reads. It was found that there were 32 differently expressed miRNAs between the two groups. In addition, 130 novel miRNAs were predicted by the Mireap Software.  Conclusion   Solexa sequencing is a rapid and effective method for discovering small RNAs and screening differentially expressed species. These miRNAs may function as regulators of many key genes during the development and reconstruction of brain injury.

Key words:  High-throughput sequencing； Cerebral ischemia reperfusion； Small