Abstract:

Objective   To construct an miR-122 expression vector and examine the effect of miR-122 expression in reversing the malignant phenotype of the HepG2.2.15 cell line. Methods   Genomic DNA of the HepG2.2.15 cell line was used as a template and the target gene fragment was PCR-amplified. The product was confirmed by enzymatic digestion and sequencing. The vector was transfected into HepG2.2.15 cells. HBsAg and HBeAg in the supernatant from cell cultures were measured, and HBV-DNA level was detected by real-time PCR. Cell proliferation after transfection was monitored. Results    The MiR-122 expression vector was expressed in HepG2.2.15 cells，leading to inhibition of HBV replication and expression as well as proliferation of HepG2.2.15 cells. Conclusion   MiR-122, expressed by means of an expression vector, inhibits HBV replication and expression. Proliferation of HepG2.2.15 cells was also impaired.

Key words:  miR-122; Expression vector; Hepatitis B virus; Carcinoma, hepatocellular; HepG2.2.15