Abstract:

Objective  To investigate the effects of endothelial cells activation as well as chemokines expression of IFN-γ inducible protein 10 (IP-10)  and IFN-γ inducible T cell α chemoattractant (I-TAC) initiated by allogeneic monocytes. Methods  Human monocytes were isolated and purified from healthy human peripheral blood mononuclear cells, endothelial cells were isolated form human aorta. A co-cultured system of endothelial cells and allogeneic monocytes was established. Endothelial cells chemokine expression of IP-10 and I-TAC were analyzed by RT-PCR before and after co-culture. The level of CD54 and CD62E on endothelial cells was detected by florescence activated cell scanning (FACS). Results  RT-PCR demonstrated that IP-10 and I-TAC mRNA levels elevated markedly at 24 hours( P<0.05), remained at high level at 48 hours and at 72 hours after co-culture（P<0.05）. FACS analysis revealed low level expression of CD54 and CD62E molecules on single cultured endothelial cells. When endothelial cells were co-cultured with allogeneic monocytes, the expression of CD54 and CD62E were significantly upregulated.（P<0.05）. Conclusion  It is suggested that immunoreaction  between allogenic monocytes and endothelial cells leads to activation of endothelial cells， which induces the expression of cell adhesion molecules and chemokines  which may play a critical role in graft rejective reaction.

Key words: Monocytes; Endothelial cells; Adhesion molecules; Chemokine; Rejection