Abstract:

Objective  To construct a recombinant adenovirus that can simultaneously express antisense ornithine decarboxylase (ODC) and S-adenosylmethionine decarboxylase (AdoMetDC) specifically in prostate cancer cells and to evaluate its tissue-specific expression. Methods  Fragments of ODC and AdoMetDC cDNAs were inserted into pMD19-T simple vector followed by ligation with PGL3- PSES fragment and then recombined with pShuttle-Basic vectors in ADxsi cells. The AdPSES-ODC-AdoMetDCas virus was produced in HEK293 cells. Western blot，cancer cell invasion and MTT analysis were applied to detect ODC and AdoMetDC expressions in tumor cells. Results  The recombinant adenovirus, packaged and amplified in HEK293 cells， was successfully constructed. Western blot，cancer cell invasion and MTT analysis results showed that expressions of ODC and AdoMetDC were significantly lower in prostate cancer cells infected with the recombinant adenovirus than in HT29 cells infected with the same virus. Conclusion  The recombinant adenovirus can specifically deplete ODC and AdoMetDC expressions in prostate cells and the decreased expressions have tissuespecificity.

Key words: Ornithine decarboxylase; Sadenosylmethionine； Prostate neoplasms