JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2010, Vol. 48 ›› Issue (6): 31-35.

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Effects of photodynamic therapy with 5-aminolevulinic acid on apoptosis of  human cervical carcinoma Hela and Siha cells in vitro

WANG Haiyan, ZHANG Youzhong, JIANG Kan, JIAO Hui, WANG Zhaojing   

  1. Department of Gynecology, Qilu Hospital of Shandong University, Jinan 250012, China
  • Received:2009-10-21 Online:2010-06-16 Published:2010-06-16

Abstract:

Objective  To study effects and mechanisms of 5-ALA-PDT(5-aminolevulinic acid- photodynamic therapy) on apoptosis of cervical adenocarcinoma Hela and squamous cell carcinoma Siha cells in vitro. Methods  MTT assay and AnnexinⅤ-FITC/PI double staining assay were applied to investigate the effects of 5-ALAPDT on proliferation and apoptosis of Hela and Siha cells respectively. Effects of 5-ALA-PDT on expression of Her-2/neu in the two cell lines were detected by immunocytochemistry. Results  Hela cells were more sensitive to 5-ALA-PDT than Siha cells. 2mmol/L 5-ALA and a light dose of 10J/cm2 were the optimal  condition for the killing of Hela and Siha cell lines in vitro by 5-ALA-PDT, under which, the 50% inhibition concentrations (IC50) of 5-ALA-PDT for Hela and Siha cell lines were 0.724mmol/L and 1.206mmol/L respectively. 5-ALA-PDT induced apoptosis of Hela and Siha cells remarkably and inhibited expression of Her-2/neu protein in the two cell lines. Conclusion  5-ALA-PDT has the patent proliferation-inhibiting and apoptosis-promoting effects on the cervical carcinoma Hela and Siha cells in vitro. The mechanism may be related to the down-regulation of Her-2/neu expression.

Key words: 5-Aminolevulinic acid; Photodynamic therapy; Apoptosis; Cervical neoplasms

CLC Number: 

  • R737.31
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