Abstract:

Objective     To study the characteristic and significance of microsatellite loss of heterozygosity(LOH) in primary foci of recurrence laryngeal squamous cell carcinoma. Methods     Allelic deletions analysis was performed on primary carcinoma and adjacent tissues in 31 cases of recurrence laryngeal squamous cell carcinoma with 3 polymorphic markers (D3S1234，D5S592 and D9s171) on 3p, 5p and 9p chromosomal arms by polymerase chain reaction-single strand conformational polymorphism(PCR-SSCP)-silver staining. Results     Occurrence rates of LOH in the three markers were 48.3%(15/31), 45.2%(14/31) and 61.3%(19/31), respectively. The patients with LOH detected had shorter recurrence time than those with no LOH detected. A statistical significance was found between the two groups(P<0.05). Conclusions    There is a negative correlation between LOH and recurrence time.  LOH has a predictive value with respect to post-operative relapse in laryngeal cancer patients. It is effectively instructive to choose the best clinical treatment protocol after operation.

Key words: Laryngeal squamous cell carcinoma; Loss of heterozygosity; Polymerase chain reaction