JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2010, Vol. 48 ›› Issue (12): 62-66.

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Construction and characteristics of E-cadherin induced cancerization model  from ovarian epithelial cells

ZHANG Hong1,  LIU Lian2,3, DONG Ling-ling2,3, DU Chao3, MA Chun-hong3, HAN Li-hui3, LI Li2   

  1. 1. Department of Geriatrics, Qilu Hospital of Shandong University, Jinan 250012, China;
    2. Department of Chemotherapy, Qilu Hospital of Shandong University, Jinan 250012, China;
    3. Institute of Immunology, School of Medicine, Shandong University, Jinan 250012, China
  • Received:2010-11-02 Published:2010-12-16

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Abstract:

Objective    To construct E-cadherin induced cancerization model from ovarian epithelial cells and make primary research on its biological behaviors such as proliferation and growth. Methods    Chinese hamster ovary (CHO) cells were transfected by plasmid including whole human E-cadherin cDNA and stably screened with G418. Expression of E-cadherin was identified by Western Blot, and cell proliferation test with CCK8 and colony formation test were used to detect their possible malignant transformation of biological behaviors. Results    A high level of E-cadherin expression, high ability of proliferation and acquisition of anchorage-independent growth were found in CHO cells transfected by E-cadherin plasmid. Conclusion     An E-cadherin induced cancerization model of ovarian epithelial cells was successfully constructed to provide a valuable tool to study the mechanisms of ovarian cancer. 

Key words: E-cadherin；Ovarian epithelial cell；Cancerization；Cell model

CLC Number: 

  • R737.31
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