Abstract:

Objective    To investigate the effects of Indobufen on platelet aggregation and thrombosis in animal models. Methods    In each study, 50 animals were randomly divided into five groups, i.e. group NS, group L(low concentration), group M(medium concentration)， group H (high concentration) of Indobufen and group ASA (Aspirin, positive control）, according to their body weight. After being treated intravenously for 30 minutes, one-minute and five-minute platelets′aggregation rate and the maximum aggregation rate, which were induced by adenosine diphosphate （ADP）, arachidonic acid（AA）or platelet activating factor（PAF）, were assayed. Moreover, the time of thrombotic paralysis or even death caused by collagen-epinephrine and the time of carotid artery thrombosis caused by electrical stimulation during the whole process were tested, too. Results    The platelet aggregation study showed that Indobufen group of M, H and ASA 22group, compared with group NS, demonstrated the ability to inhibit ADP, AA and PAF-induced platelet aggregation at different time points（P<0.05 or P<0.01）. In the inducement study of ADP and AA, compared with group ASA, Indobufen group of H showed a significant discrepancy in marked inhabitation of the one-minute/five-minute platelet aggregation rate（P<0.05）. Based on the pulmonary embolism study induced by collagen  adrenaline in mice, the survival time of the mice in the Indobufen groups of M， H and group ASA increased much more than group NS（P<0.01 or P<0.05）. Meanwhile, the survival time of group H was considerably longer than group ASA（P<0.05）. According to the results of the carotid artery thrombosis study, Indobufen groups of M，H and group ASA could significantly inhibit the thrombosis time（P<0.05 or P<0.01）， compared with group NS. Although the thrombosis time inhibited by Indobufen did not show a significant difference, Indobufen group of H presented a longer time to inhibit thrombosis than group ASA. Conclusion     Indobufen is a very potential inhibitor of platelet aggregation and thrombus formation in vivo, and the effect is dose-dependent. To inhibit the platelet aggregation and thrombosis, Indobufen is better than ASA.

Key words: Indobufen; Platelet aggregation; Thrombosis; Rats， Wistar; Mice， KM