Abstract:

Objective    To investigate the influence of JAK inhibitor AG490 on proliferation and apoptosis of human laryngeal cancer Hep-2 cells, reveal the inhibitory effect of AG490 on the JAK/STAT3 signaling pathway,  and further explore the potential mechanism so as to study the role of AG490 in the treatment of laryngeal carcinoma. Methods    Hep-2 cells cultured in RPMI1640 medium were treated with JAK inhibitor AG490 at different time. The state of cell proliferation was detected by MTT assay, and the apoptosis and cell cycle of Hep-2 were analyzed by flow cytometry(FCW). Expressions of STAT3 and pSTAT3 proteins were detected by Western blot. Results     AG490 inhibited cell proliferation in a dose-and time-dependent manner. The apoptosis cells increased with the increase of action time. AG490 resulted in a significant down-regulation in STAT3 and pSTAT3 expressions in the Hep2 cell line. Conclusion    AG490 can significantly inhibit proliferation and induce apoptosis of laryngeal cancer Hep-2 cells through decreasing expressions of STAT3 and p-STAT3.

Key words: Laryngeal neoplasms; Signal transducers and activators of transcription 3; Proliferation; Apoptosis; JAK inhibitor