Abstract:

Objective     To evaluate the capacities of insulin glargine and aspart  to normalize the blood glucose profile in diabetic NOD mice. Methods      Thirty newly diagnosed diabetic female NOD mice were randomized into A,B,C,D and E groups(in each group, n=6). Group A，B , C and D had free access to food and insulin glargine(IG) were given at 9:00 subcutaneously in corresponding doses: 0.1IU/g·d-1、0.05IU/g·d-1、0.015IU/g·d-1 and PBS 5μL·d-1. Intensive insulin therapy was applied in group E, in which insulin glargine, as a basal replacement, was administrated subcutaneously at 9:00, and insulin aspart(IA) was administrated subcutaneously every four hours( beginning at 03：00 every day ) followd by one-hour feeding  Meanwhile, another group, i.e.group F(n=6), with the ages parallel to non-diabetic NOD mice, was included in the study. The blood glucose value at different time points (24 hrs) on the fourth day were recorded and the effects of each treatment were analyzed on the 15th day.  Results      The average blood glucose was significantly lower in group E than Group B,C and D(P≤0.05, respectively). But there was no significant difference found between Group E and Group A/ F(P>0.05). The fluctuation of blood glucose in Group E was significantly smaller than those in group A, B and C(P<0.05, respectively). The percentage of normal blood glucose during 24 hours in group E was much higher than those in group B, C and D(P<0.05, respectively). Severe hypoglycemia and hyperglycemia were often observed in those single insulin glargine groups. The symptoms of diabetes was under control after 15 days of insulin treatment,however, there were 5 and 3 deaths in group A and B due to hypoglycemia. Conclusions    Intensive therapy with preprandial IA plus basal IG is superior than once daily basal IG to achieve a better glycemic control. 

Key words: Disease model； Type 1 diabetes； Blood glucose； Insulin； Mice,  aminal