JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2010, Vol. 48 ›› Issue (11): 70-.

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Effects of sodium butyrate on cell proliferation and expression of ICAM-1 in the HT-29 colon cancer cell line

LI Wan-quan1, ZHU Xiao-lin2, YU Cheng-gong2   

  1. 1. Xinxiang Hongqi Hospital, Xinxiang 453100, Henan, China;  2. Department of Gastroenterology, The Affiliated Drum
    Tower Hospital of Nanjing University  Medical School, Nanjing 210008, China
  • Received:2010-05-15 Online:2010-11-16 Published:2010-11-16

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Abstract:

Objective    To investigate the effects of sodium butyrate on HT-29 colon cancer cell proliferation and expression of intercellular adhesion molecule 1 (ICAM-1) on the cell surface， and to explore the mechanism by which sodium butyrate affects growth of HT-29 cells. Methods    Effect of sodium butyrate on the proliferation of HT-29 cells was detected by MTT［1-(4,5-Dimethylthiazol-2yl)-3,5-diphenylformazan］ assay. The cell cycle, cell apoptosis and expression of ICAM-1 induced by sodium butyrate were measured by flow cytometry. Results    In a time-and dose-dependent manner， sodium butyrate significantly inhibited the growth of HT29 cells, increased the proportion of G0/G1 cells and the proliferation index, decreased the proportion of S phase cells， and induced cell apoptosis. Also, sodium butyrate down-regulated expression of ICAM-1. Conclusion    Sodium butyrate can inhibit the proliferation of HT-29 cells. The mechanism may relate to arresting the cell cycle, inducing cell apoptosis and down-regulating expression of ICAM-1 on HT-29 cells.

Key words: Sodium butyrate; HT-29; Colon cancer; Intercellular adhesion molecule 1

CLC Number: 

  • R735.3
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