Abstract:

Objective     To research the cell tropism of enterovirus 71(EV71) using sensitized sheep erythrocytes and explore expression of the EV71 receptor on the surface of passage culture cells.  Methods     Sheep erythrocytes sensitized with EV71 reacted with passage culture cells, and the ability of the cultured cells treated with different methods to absorb the sensitized sheep erythrocyte was observed. Then the cell tropism of EV71 was analyzed and expression of the EV71 receptor on the surface of the cultured cells was explored. Results     Human laryngeal epithelial (Hep-2) cells and rhesus monkey embryo kidney(MA104) cells absorbed the sensitized sheep erythrocytes and the trypsin destroyed the absorption ability of MA104 but not of Hep-2. The absorption of sensitized sheep erythrocytes was not observed in Madin-Darby canine kidney (MDCK) cells. All results agreed well with the results of the EV71 direct infection. Conclusion     This method  proved to be direct and effective to research the cell tropism of virus and expression of the virus receptor on the surface of the cells, and it also has strong feasibility and application value in the research of virology, especially of virus receptors.

Key words: Enterovirus A, human； Receptor, virus； Sheep erythrocyte； Cell tropism