Abstract:

To investigate the relationship between DDR2 and alcoholic liver fibrosis as well as the response to compound glycyrrhizin tablets treatment on them. Methods48 rats were randomly divided into 6 groups (8 in each group): the control group, the model group, the Alc disc group, the Alc contin group, the CGT + Alc disc group and the CGT + Alc contin group. After 16week intragastric administration with alcohol, rats in the model group were sacrificed, at the same time rats in the control group were killed. The rest having been treated by intrastric alcoholic for 16 weeks in the Alc disc group (stopped alcohol intragastric), the CGT + Alc disc group (stopped alcohol intragastric and treated by CGT), the Alc contin group (continued alcohol intragastric without treatment of CGT) and the CGT + Alc contin group (continued alcohol intragastric with treatment of CGT simultaneously) were sacrificed at the end of the 20th week. Levels of serum hyaluronic acid (HA), laminin (LN), procollagen type Ⅲ (PCⅢ) and collagen type Ⅳ (CⅣ) were determined. Pathologic changes of the liver were observed by HE staining and Masson staining. Expressions of DDR2 mRNA and protein were determined by RTPCR and Western blot, respectively. ResultsPathological liver fibrosis was obviously found in rats with alcoholic intragastric administration for 16 weeks. Areadensity percentage of collagen fibrosis, levels of serum HA, LN, PCⅢ and CⅣ, and levels of DDR2 mRNA and protein in the liver were significantly increased in these indexes were decreased in the CGT + Alc disc group compared with the Alc disc group and in the CGT + Alc contin group compared with the Alc contin group(P＜0.05). ConclusionCGT had certain therapeutic effects on alcoholic liver diseases, which may relate to downregulation of DDR2 expression.

Key words:  Liver cirrhosis, alcoholic; Compound glycyrrhizin tablet; Discoidin domain receptor 2