Abstract:

ObjectiveTo study the immunological reaction of mice with mucopolysaccharidosis treated by human fetal liver cell transplantation in order to select an  immunosuppressive agent. MethodHuman fetal liver cells(FLCs) were transplanted into αLduronidase (IDUA) deficiency mice pretreated with different immunosuppressive agents. T cell subsets（CD3,CD4/CD8）were detected by flowcytometry, while cytokines(IgG,IgM,IL2, TNFα and IFNγ) by EL ISA, and serum  IDUA activity by a fluorospectrophotometer at different stages (before and after transplantation). ResultsThe T cell subsets and cytokines in recipients were significantly increased after FLCs transplantation. Five days after transplantation, IDUA activity reached a peak in all groups (P＜0.01), then gradually decreased. The IDUA activity lasted the longest in the group pretreated with cyclophosphamide cyclosporine FTY720 and predinisone. ConclusionIDUA activity can be raised by FLCs transplantation. Due to immunological rejection, the recipient should be given immunosuppressive therapy. Cyclophosphamide cyclosporine FTY720 in combination with predinisone can give the best immunosuppression.

Key words: Fetal liver cells; Transplantation; Mucopolysaccharidosis; Immunosuppressive agent