Abstract:

To harvest hFGF7 modeling peptides from a phage random twelve peptide library to promote epidermal cells proliferation. MethodsA phage random twelve peptide library was screened 4 times by monoclonal antihuman FGF7. ELISA staining examination was performed to choose monoclonal phages at random. Also, DNA extracted from phages with better bindingactivity was sequenced and genecompared. ResultsAfter 4 times of screening, abundant of specific phage modeling peptides were harvested. The results of ELISA showed a fine associativity of some phage modeling peptides. A total of 11 sequences were recognized which were related to promoting cell division and proliferation or inhibiting cell proliferation. ConclusionhFGF7 related phage modeling peptides can be harvested from a phage random twelve peptide library， in which some phage modeling peptides may promote epidermis cells proliferation.

Key words: Phage random twelve peptide library; Homo fibroblast growth factor7 phage modeling peptide; Screen; Sequence analysis