Abstract:

To observe the effect of myeloperoxidase (MPO) on monocyte chemoattractant protein 1(MCP1) expression in endothelial cells and the intervention of metformin. MethodsMCP1 and AMPKα protein expressions in endothelial cells were determined by the westernblot method, and the level of MCP1 in  culture medium was measured by the sandwich ELISA method. Also, RNA interference technology was used to determine the role of AMPKα. ResultsBoth MCP1 protein expression in endothelial cells and content of MCP1 in culture medium were significantly increased by MPO in a dose and timedependent manner（P＜0.01）, and metformin decreased  MCP1 protein expression and content of MCP1 by promoting phosphorylation of AMPKα, however, AMPKαSiRNA significantly weakened the effect of metformin（P＜0.01）. ConclusionMetformin can inhibit the effect of MPO on endothelial cell MCP1 protein expression and MCP1 concentration.

Key words:  Monocyte chemoattractant protein1; Endothelial cells; Myeloperoxidase; Metformin; AMPactivated protein kinase alpha