Abstract: To establish the comparative proteomic technology platform for Demyelinative disease of the central nervous system in cerebrospinal fluid and to screen out disease related protein markers by proteome analysis. MethodsProteins and peptides in CSF were separated by twodimensional gel electrophoresis(2DE) and ultraperformance liquid chromatographer(UPLC) and identified by matrix assisted laser desorption ionization time of flight mass spectrometry（MALDI-TOF-MS）and electrospray ionization(ESI-MS) to study differential proteomic expression in the cerebrospinal fluid(CSF) between patients with Demyelinative disease (the experimental group) and with other neurological diseases (ONDs) (the control group). ResultsDifferent 2DE maps were obtained for the protein spots in the experimental group and the control group gels. Image analysis of silverstained 2DE gels revealed that 10 protein spots showed a significantly differential expression between the two groups of CSF samples. After searching the database, it was found that in the CSF of Demyelinative patients, the expression of hemoglobin was increased. However, the expression of ProstaglandinH2 Disomerase was decreased.Conclusion These identified proteins have potential relations to Demyelinative disease physiology. It would be helpful to find new molecular markers for early diagnosis and treatment of the disease.

Key words: Neuroproteomics, Twodimensional gel electrophresis, Cerebrospinal fluid, Demyelinative disease