卵巢癌组织及细胞株中肿瘤特异性抗原MAGE、GAGE、BAGE基因表达的研究

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)

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Expression of tumor specific antigen MAGE, BAGE, GAGE in ovarian cancer tissues and cell lines

ZHANG Shi-qian¹，ZHOU Xiao-liang²，ZHANG Lin-lin¹，WU Xi-mei³，SHI Yan⁴

1. Department of Gynecology, Qilu Hospital of Shandong University；2. Department of Gynecology, First Affiliated Hospital of Henan University of Science and Technology；3. Shandong Provincial Institute of Family Planning Research

Received:2007-01-17 Revised:1900-01-01 Online:2007-08-24 Published:2007-08-24
Contact: ZHANG Shi-qian

Abstract

Abstract: Objective: To explore the expression of MAGE, BAGE and GAGE genes in ovarian cancer tissues and cell lines. Methods: Expression levels of MAGE-1, MAGE-3, GAGE-1/2 and BAGE were determined by reverse transcription polymerase chain reaction(PTPCR) in ovarian cancer cell lines SKOV3, A2780, COC1, and 10 cases of normal ovarian tissues, 20 cases of benign epithelial tumor, and 47 cases of malignant epithelial tumor. Results: Normal ovarian tissues did not express members of the above gene families. In benign tumor tissue specimens, only 3 cases expressed MAGE-1 (3/20). 16(34%)， 12(25.5％), 7(14.9％) cases of 47 ovarian cancer tissues. Conclusion: High expression of MAGE, BAGE and GAGE genes is produced in ovarian cancer tissues, which is related to the tumor grade, clinical stage, ascites, and the histological subtype, but is not related to age and lymph node metastasis. There was at least one of these genes expressed in ovarian cancer cell line SKOV3, A2780, and COC1.These tumor-specific antigens can be used as molecular markers for diagnosis and possible targets for immunotherapy.

Key words: Ovarian neoplasms, Tumor specific antigen, MAGE gene, BAGE gene, GAGE gene

CLC Number:

R737.31

ZHANG Shi-qian,ZHOU Xiao-liang,ZHANG Lin-lin,WU Xi-mei,SHI Yan. Expression of tumor specific antigen MAGE, BAGE, GAGE in ovarian cancer tissues and cell lines[J].JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2007, 45(8): 762-766.

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