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Inhibitory effect of decarboxylase and S-adenosylmethionine decarboxylase bi-antisense virus on lung cancer cell A-549

SUN Qi-feng1，TIAN Hui1，LIU Xian-xi2，ZHANG Bing2，ZHANG Yan2，SUN Dong-feng1   

  1. 1． Department of Thoracic Surgery, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China;2. Experimental Center of Medical Molecular Biology, School of Medicine, Shandong University
  • Received:2007-04-11 Revised:1900-01-01 Online:2007-08-24 Published:2007-08-24
  • Contact: TIAN Hui

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Abstract: Objective: To study the inhibitory effect of ornithine decarboxylase(ODC) and S-adenosylmethionine decarboxylase bi-antisense AdoMetDCas (Ad-ODC-AdoMetDCas) virus on polyamine biosynthesis, proliferation and invasion of lung cancer cells. Methods: Adenovirus-mediated gene transduction efficiency was assessed by counting positive GFP cells using FACS. The malignant phenotype of A-549 cells was assessed by growth curves. Western Blot and HPLC were used to determine the expression of ODC and AdoMetDC and the polyamine content in A-549 cells, respectively. TUNEL was used to analyze cell apoptosis. Results: Approximate 75% of A-549 cells were infected with Ad-ODC-AdoMetDCas when MOI reached 50. Expression of ODC was inhibited in the infected tumor cells. Ad-ODC-AdoMetDCas inhibited growth and invasive ability of A-549 cells. TUNEL proved that Ad-ODC-AdoMetDCas could lead to cell apoptosis. Ad-ODC-AdoMetDCas vector-mediated gene transfer inhibited tumor cell growth through the blockade of the polyamine synthesis pathway. Conclusion: Ad-ODC-AdoMetDCas has a significant inhibitory effect on proliferation and invasion of lung cancer cells and has therapeutic potential for the treatment of lung cancer.

Key words: Ornithine decarboxylase, S-adenosylmethionine decarboxylase, Polyamine, Lung neoplasms, Gene therapy

CLC Number: 

  • Q78
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