Abstract: ObjectiveTo investigate the effects of glutamine on the expression of heat shock protein (HSP) 70 in pulmonary micro-vascular endothelial cells (PMVECs) under oxidative stress conditions. MethodsPMVECs from Wistar rats were divided into 5 groups: the positive control group (G group), the physiologic saline group (PS group), the glutamine group(Gln group), the H₂O₂+PS group and the H₂O₂+Gln group. Western blotting was used to investigate the expression of HSP70. ResultsCompared with the PS group, the expression of HSP70 significantly increased in the Gln group（9.05±1.51 vs 40.28±10.19） and the H2O2+PS group（9.05±1.51 vs 43.89±10.32）. The expression of HSP70 was higher in the H₂O₂+Gln group than in the H₂O₂+PS group（65.59±14.53 vs 43.89±10.32）. ConclusionGlutamine enhances the expression of HSP70 in pulmonary micro-vascular endothelial cells under oxidative stress conditions.

Key words: Pulmonary microvascular endothelial cells, Hydrogen peroxide, Glutamine, HSP70 heat-shock proteins