Abstract: Objective: To explore the role of HPV16/E7, the cellular proliferation activity and the tumor angiogenesis in laryngeal papilloma(LP) in adults. Methods: Twenty tissues of LP in adults were selected in the experimental group, and 14 tissues of vocal cord polyp (VCP) and 14 tissues of laryngeal squamous cell carcinoma(LSCC) were enrolled in the control groups. Expression of HPV16/E7 was determined by the polymerase chain reaction(PCR) method, and of Ki-67 and CD34 by the immuneohistochemical SP method. Results: The expression rate of HPV16/E7 in VCP, LP and LSCC was 14.3%, 66.7%, and 85.7%, respectively. The labeling index(LI) of Ki-67 was 6.30±2.03, 13.73±3.42, and 25.55±3.92, respectively. The number of MVD was 3.97±1.38,14.61±1.64, and 25.34±2.63, respectively. The difference was significant (P＜0.01). The level of HPV16/E7, CD34 was positively correlated with that of Ki-67 in the tissues（r=0.854,P＜0.01; r=0.878,P＜0.01）. Conclusion: Ki-67 and MVD highly correlates with the tumor angiogenesis, cellular proliferation activity and pathological types, and they can be served as the malignant indicators for LP in adults.

Key words: Laryngeal neoplasms, Laryngeal papilloma, Proliferating cell antigen, CD34