Abstract: To assess and compare the potenti of mouse immunecostimulating signal B71 and B72 vaccine in inducing antitumor immunity effect. Methods: Reverse transcription expressing vector pLXSNmB71 and pLXSNmB72 were constructed to transfect wild EL4 cells, then mB71 gene and mB72 gene were introduced into EL4 cells, the positive clone (EL4/mB71 and EL4/mB72) highly expressing mB71 and mB72 molecular was selected. In vitro, The secretion of IL2 of EL4/mB71 and EL4/mB72 vaccine stimulatedlymphocytes, and the T cell functions of proliferation and cytotoxicity were detected by the method of LDHrelease assay, MTT assay and ELISA. Results: ① IL2 production stimulated by EL4/mB71 and EL4/mB72 vaccine was much higher than wild EL4 cells（P＜0.01）; ② IL2 production stimulated by EL4/mB71 vaccine was higher than EL4B72 vaccineat 24 hours (P＜0.05), whereas IL2 production stimulated by EL4/mB72 vaccine was higher than EL4/mB71 vaccine at 48 hours (P＜0.05); ③ In vitro, both mB71 vaccineactivated CTL and mB72 vaccineactivated CTL exhibited a specific cytotoxicity. These two kinds of activated CTL had no significant difference in killing tumor cells (P＞0.05). Conclusion: Both mB71 and mB72 can activate T cell to produce IL2, which get to the peak not at the same time. mB71 and mB72activated CTL exhibit the similar specific cytotoxicity.