Abstract: To establish a method of culture of retinal neuron cells in vitro.　Methods: Cell suspension was made from retina of postnatal 1～3 day Wistar rat by using trypsin and ethylene diamion tet raacetic acid (EDTA ) digestion, and then it was planted to cover slips covered with PolyLlysine in 24 well plates. The growth regularity of cells in vitro was observed under the phase contrast microscope, and the cells were identified by using immunohistochemistry, and then the NFpositive ones were counted under the light microscope. Results： Retinal neuron cells cultivated in PolyLlysine grew very well and some possessed axons, of which some were connected with each other. Most cells were NF positive detected by immuniohistochemistry. Trypsin and ethylene diamion tet raacetic acid (EDTA) had salutary effect for making retina tissues into cell suspension. Conclusion： Cultivation of retinal neuron cells using enzyme digestion well develops in vitro. Trypsin combined with ethylene diamion tet raacetic acid (EDTA) is better than trypsin alone for digestion of epithelium in neurosensory retina.

Key words: Retina, Neuron cells, Cell culture, Antigen NF