Journal of Shandong University (Health Sciences) ›› 2019, Vol. 57 ›› Issue (1): 41-47.doi: 10.6040/j.issn.1671-7554.0.2018.468

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Effects of orexin receptors on the spatial learning and memory and proliferation of dentate gyrus cells in rats with sleep deprivation

MIAO Suyun1,2, NI Liyan1,3, WANG Li1,4, SONG Limin1, TANG Jiyou1   

  1. 1. Department of Neurology, Qianfoshan Hospital Affiliated to Shandong University, Jinan 250014, Shandong, China;
    2. Department of Neurology, Central Hospital of Taian, Taian 271000, Shandong, China;
    3. People's Hospital of Juye County, Juye 274900, Shandong, China;
    4. Department of Traditional Chinese Medicine andWestern Medicine, Yuhuangding Hospital of Yantai, Yantai 264000, Shandong, China
  • Published:2022-09-27

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Abstract: Objective To investigate the effects of lentivirus mediated siRNA silencing hippocampal orexin-1 receptor(OX1R)and orexin-2 receptor(OX2R)genes on the spatial learning and memory and the proliferation of dentate gyrus cells in sleep-deprived rats. Methods A total of 40 healthy adult male Wistar rats were screened and randomly divided into cage control group, treadmill control group, sleep deprivation group, lentivirus group and lentivirus mediated group, with 8 rats in each group. Rats in the sleep deprivation, lentivirus and lentivirus-mediated groups ran intermittently on treadmills for 72 hours for sleep deprivation. The spatial learning and memory ability of all rats was investigated with Morris water maze. The numbers of bromodeoxyuridine-labelled(BrdU+)cells, and BrdU+/NeuN+ cells were quantified with a confocal laser-scanning microscope. Results Compared with the treadmill control group, the sleep deprivation group showed significantly prolonged escape latency, decreased times across platform, and reduced numbers of BrdU+ and BrdU+/NeuN+ cells(P<0.05). Compared with the lentivirus group, the lentivirus-mediated group showed shortened escape latency, increased times across platform(P<0.05), and increased numbers of BrdU+ and BrdU+/NeuN+ cells(P< 山 东 大 学 学 报 (医 学 版)57卷1期 -苗素云,等. Orexin受体功能在睡眠剥夺大鼠空间学习记忆和海马齿状回细胞增殖中的作用 \=-0.05). No significant differences were observed between the lentivirus group and sleep deprivation group(P>0.05). Conclusion Sleep deprivation can impair the spatial learning and memory, and inhibit the proliferation of cells in dentate gyrus. Lentiviral mediated siRNA silencing of OX1R/OX2R genes can improve the spatial learning and memory ability of sleep-deprived rats, which may be related to the proliferation and maturation of neural cells in dentate gyrus.

Key words: Sleep deprivation, Orexin receptor, Hippocampus, Learning and memory, Neurogenesis

CLC Number: 

  • R741
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