Journal of Shandong University (Health Sciences) ›› 2018, Vol. 56 ›› Issue (12): 19-25.doi: 10.6040/j.issn.1671-7554.0.2018.338

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Effect of adipose-derived stem cells on intrinsic skin aging

ZHANG Kun1,2, YAN Bing3, LI Fang1,2, XIAO Dongjie1,2, WANG Yunshan1,2, LIU Hua1,2   

  1. 1. Laboratory of Cell Therapy and Translational Medicine, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, Shandong, China;
    2. Shandong Research Center of Transplantation and Tissue, Jinan 250013, Shandong, China;
    3. Department of Gastrointestinal Surgery, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, Shandong, China
  • Published:2022-09-27

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Abstract: Objective To observe the effects of adipose-derived stem cells(ADSCs)on intrinsic skin aging, and investigate the underlying mechanisms. Methods Mouse skin fibroblast cells(MSFCs)and human ADSCs were primarily cultured. MSFCs cells were randomly divided into control group, model group, ADSCs conditioned medium(ADSCs-CM)group. MSFCs were treated with D-galactose(D-gal)and ADSCs-CM. The changes of ROS and β-galactosidase were tested by microscopy and flow cytometry, the expressions of caspase3, p53 and sirt1 were detected by RT-PCR, and the expression of Sirt1 protein was detected by Western blotting. Results The level of ROS in model group was higher than that in control group(P<0.001), and the β-galactosidase staining was deepened. The level of ROS in ADSCs-CM group was lower than that in model group(P<0.001), and the β-galactosidase staining was lighter. RT-PCR showed that the expressions of caspase3(P<0.001)and p53(P=0.001)were increased while the expression of sirt1(P<0.001)was decreased in model group compared with those in control group. The expressions of caspase3 山 东 大 学 学 报 (医 学 版)56卷12期 -张坤,等.脂肪源性干细胞对内源性皮肤老化的治疗作用 \=-(P<0.001)and p53(P=0.001)were decreased while the expression of sirt1 (P<0.001)was increased in ADSCs-CM group compared with that in model group. Western blotting showed that the Sirt1 protein expression was decreased in model group compared with that in control group(P<0.001)and increased in ADSCs-CM group compared with that in model group(P=0.006). Conclusion ADSCs show a protective effect on intrinsic skin aging induced by D-gal via Sirt1 signal pathway.

Key words: Adipose-derived stem cells, D-galactose, Skin aging

CLC Number: 

  • R751
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