Journal of Shandong University (Health Sciences) ›› 2019, Vol. 57 ›› Issue (5): 67-73.doi: 10.6040/j.issn.1671-7554.0.2018.1486

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Effects of lentivirus injection via testicular efferent tubules on the reproductive system in Wistar rats

DI Yu, CHEN Dongshan, YAN Lei   

  1. Department of Urology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Published:2022-09-27

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Abstract: Objective To investigate the effect of stably transgenic technology of highly toxic lentivirus on the reproductive system of male Wistar rats. Methods The stable interference lentivirus of PAX2 containing green fluorescent protein were constructed. The solution was labeled with Trypan blue. The testicular efferent tubules injections of 16 Wistar rats were carried out with a 34 G needle. The testis parenchyma was monitored by ultrasound after two weeks. The rats were executed after one month. The testis and the accessory glands were removed and fixed, and the tissue structure and morphology were studied by immunofluorescence staining and HE staining. Results Eight rats were successfully injected. Apoptosis and necrosis in testicular convoluted seminiferous tubules were observed in different degrees. High expression of the reporter gene was observed in some tubules, and no obvious transfection was found in the epididymal epithelium. However, there was a high expression of the green fluorescent protein in seminal vesicle glandular epithelial cells. Conclusion Testis and epididymis are not suitable for lentivirus transfection in male animals for making transgenic animals, and the local transfection can easily affect sperm maturation and development. However, it is practical to establish transgenic animals by injecting lentivirus into the seminal vesicle.

Key words: Testicular efferent tubules, Lentivirus, Transgenic, Seminal vesicle, Epididymis

CLC Number: 

  • Q492.4
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