Journal of Shandong University (Health Sciences) ›› 2018, Vol. 56 ›› Issue (10): 72-78.doi: 10.6040/j.issn.1671-7554.0.2017.1194

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Effects of Notch1 on neural stem cell differentiation induced by all-trans retinoic acid

XU Jiaming, MA Aihua, CHEN Na, HAN Bo   

  1. Department of Pediatrics, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China
  • Received:2017-12-04 Published:2022-09-27

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Abstract: Objective To investigate the role of Notch1 in neural stem cell differentiation induced by all-trans retinoic acid and to reveal its effects on neural tube development. Methods The expressions of Notch1 in nestin, neurofilament(NF), glial fibrillary acidic protein(GFAP)and galactocerebroside(GALC)were identified with immunofluorescence. The neural stem cells were cultured in normal differentiation medium and the all-trans retinoic acid(ATRA)medium. The expressions of nestin, NF, GFAP and GALC on day 1, 3, 5 and 7 were detected with Western blotting. The neural stem cells were divided into 4 groups and treated with normal differentiation medium(group A), normal differentiation medium + DMSO(group B), normal differentiation media + DMSO + 25 μmol/L γ-secretase inhibitor(group C), and normal differentiation media + DMSO + 50 μmol/L γ-secretase inhibitors(group D). The expressions of Notch1 in these groups were detected with Western blotting. Results Nestin, NF, GFAP and GALC were positively expressed, and Notch1 distributed in the cell membrane and cytoplasm. In the normal differentiation medium, Notch1 山 东 大 学 学 报 (医 学 版)56卷10期 -徐加明,等.Notch1在全反式维甲酸诱导的神经干细胞分化中的作用 \=-significantly increased on day 5 compared with day 1, NF decreased significantly on day 5 compared with day 3, GFAP and GALC significantly decreased on day 5 compared with day 1. In the ATRA medium, Notch1 significantly reduced on day 7 compared with day 1, whereas NF, GFAP, and GALC significantly increased on day 7. In after adoling the γSI, the intracellular domain(NICD)of Notch1 decreased significantly. Conclusion Notch1 signal pathway inhibits neural stem cell differentiation, while ATRA promotes neural stem cell differentiation by inhibiting Notch1.

Key words: Neural tube defect, All-trans retinoic acid, Notch1, Neural stem cell

CLC Number: 

  • R729
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