JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (2): 1-5.doi: 10.6040/j.issn.1671-7554.0.2015.799

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Expression and significance of CCL25/CCR9 in chronic rejection of cardiac allografts in rats

HU Lianlong, SONG Guangmin, ZHAO Xin, BAI Xiao, ZHANG Jian, ZHAO Tinglei, WANG Long   

  1. Department of Cardiovascular Surgery, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2015-08-22 Online:2016-02-10 Published:2016-02-10

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Abstract: Objective To investigate the expression and significance of CC chemokine ligand 25(CCL25)and CC chemokine receptor 9(CCR9)in chronic rejection of cardiac allografts in rats. Methods Inbred Wistar rats and inbred SD rats were selected as donors and recipients. Cervical heterotopic heart transplantation was performed with “cuff technique”. The recipients were divided into 3 groups randomly, with 10 rats in each group: CsA group, CsA+IgG group, and CsA+anti-CCL25 group. Au vecipients were received 10 mg/kg cyclosporin A(CsA)immediately after the surgery, then once every other day for 10 times. CsA+IgG group was injected with 0.1 mg/kg IgG isotype Ab immediately after the surgery, then once every other day for 10 times. CsA+anti-CCL25 group was injected with 0.1 mg/kg anti-rat CCL25 Ab immediately after the surgery, then once every other day for 10 times. All allografts were harvested on the 70th day postoperatively. HE and sirus red staining were used to observe the pathologic changes of the allograft myocardia. Additionally, immunohistochemistry and Western blotting were adopted to detect the expressions of CCL25 and CCR9 in the myocardium. Results The myocardial inflammatory response, fibrosis and cardiac allograft vasculopathy(CAV)in CsA group and CsA+IgG group were significantly severer than in CsA+anti-CCL25 group(P<0.05); 山 东 大 学 学 报 (医 学 版)54卷2期 -胡连龙,等.CCL25/CCR9在大鼠心脏移植慢性排斥反应中的表达及意义 \=-CCL25 and CCR9 were expressed in the cardiac allografts; the expression of CCL25 and CCR9 in CsA+anti-CCL25 group was significantly lower than that in the other two groups(P<0.05). Conclusion CCL25 and CCR9 are expressed in the chronic rejection of cardiac allografts in rats. Anti-CCL25 Ab can significantly alleviate the pathologic changes of cardiac allografts through targeted blocking of CCL25/CCR9. Our study indicates that CCL25/CCR9 plays important roles in the chronic rejection of cardiac allografts in rats.

Key words: Cardiac transplantation, Chronic rejection, CC chemokine ligand 25, CC chemokine receptor 9

CLC Number: 

  • R737.1
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