JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (2): 33-37.doi: 10.6040/j.issn.1671-7554.0.2015.705

Previous Articles     Next Articles

Synapse-protective effect of flavonoids extracted from the leaves of Diospyros kaki in APP/PS1 transgenic mice

SHANG Yuying, MA Yingjuan, WU Xiaofan, HOU Xunyao, LUO Dingzhen, CHEN Jian, HONG Yan, SHEN Chao, LIU Xueping   

  1. Department of Senile Neurology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China
  • Received:2015-07-27 Online:2016-02-10 Published:2016-02-10

PDF (PC)

150

Abstract: Objective To investigate the effect of flavonoids extracted from the leaves of Diospyros kaki(FLDK)on cognition levels, synapse structure and the expression of synapse-related proteins in APP/PS1 transgenic mouse. Methods Twenty APP/PS1 mice of four-month old were randomly divided into Alzheimers disease(AD)model group(AD group)and treatment group(AD+FLDK group), ten in each group; ten C57BL/6 mice of four-month old were regarded as normal control group(NC group). AD+FLDK group was treated intragastrically with 80 mg/(kg·d)FLDK, while AD and NC groups were fed intragastrically with the same volume of normal saline. Morris water maze was used to test the escape latency(EL)and number of crossing, transmission electron microscope was used to observe the ultrastructure of synapse, and immunohistochemistry was used to detect the expression levels of synaptophysin and drebrin. Results Compared with NC group, the EL was prolonged, crossing numbers decreased, the synapse ultrastructure was damaged and synapse-related protein levels decreased in AD group. Compared with AD group, EL decreased and crossing number increased obviously, synapse ultrastructure was clearer and synapse-related protein levels increased in AD+FLDK group. Conclusion FLDK can improve cognitive decline in APP/PS1 transgenic mouse, protect the integrity of synapse, and increase the synapse-related protein levels, thus can enhance cognition levels and protect synapse.

Key words: Flavonoids, Cognition, Diospyros kaki, APP/PS1, Synapse

CLC Number: 

  • R741.05
[1] Lin N, Pan XD, Chen AQ, et al. Tripchlorolide improves age-associated cognitive deficits by reversing hippocampal synaptic plasticity impairment and NMDA receptor dysfunction in SAMP8 mice[J]. Behav Brain Res, 2013, 258(2014):8-18.
[2] Oliveira DR, Sanada PF, Saragossa FA, et al. Neuromodulatory property of standardized extract Ginkgo biloba L.(EGb 761)on memory:behavioral and molecular evidence[J]. Brain Res, 2009, 1269:68-89.
[3] Walker JM, Klakotskaia D, Ajit D, et al. Beneficial effects of dietary EGCG and voluntary exercise on behavior in an Alzheimers disease mouse model.[J]. J Alzheimers Dis, 2015, 44(2):561-572.
[4] Rendeiro C, Vauzour D, Kean RJ, et al. Blueberry supplementation induces spatial memory improvements and region-specific regulation of hippocampal BDNF mRNA expression in young rats[J]. Psychopharmacology(Berl), 2012, 223(3):319-330.
[5] Li H, Wu X, Wu Q, et al. Green tea polyphenols protect against okadaic acid-induced acute learning and memory impairments in rats[J]. Nutrition, 2014, 30(3):337-342.
[6] Williams CM, El Mohsen MA, Vauzour D, et al. Blueberry-induced changes in spatial working memory correlate with changes in hippocampal CREB phosphorylation and brain-derived neurotrophic factor(BDNF)levels[J]. Free Radic Biol Med, 2008, 45(3):295-305.
[7] Bei W, Peng W, Zang L, et al. Neuroprotective effects of a standardized extract of Diospyros kaki leaves on MCAO transient focal cerebral ischemic rats and cultured neurons injured by glutamate or hypoxia[J]. Planta Med, 2007, 73(7):636-643.
[8] Gandhi S, Abramov AY. Mechanism of oxidative stress in neurodegeneration[J]. Oxid Med Cell Longev, 2012:428010.
[9] Rao JS, Kellom M, Kim HW, et al. Neuroinflammation and synaptic loss[J]. Neurochem Res, 2012, 37(5):903-910.
[10] Selkoe DJ. Alzheimers disease is a synaptic failure[J]. Science, 2002, 298(5594):789-791.
[11] Trinchese F, Liu S, Battaglia F, et al. Progressive age-related development of Alzheimer-like pathology in APP/PS1 mice[J]. Ann Neurol, 2004, 55(6):801-814.
[12] Yu YF, Zhai F, Dai CF, et al. The relationship between age-related hearing loss and synaptic changes in the hippocampus of C57BL/6J mice[J]. Exp Gerontol, 2011, 46(9):716-722.
[13] Lin YC, Yeckel MF, Koleske AJ. Abl2/Arg controls dendritic spine and dendrite arbor stability via distinct cytoskeletal control pathways[J]. J Neurosci, 2013, 33(5):1846-1857.
[14] Aoki C, Sekino Y, Hanamura K, et al. Drebrin A is a postsynaptic protein that localizes in vivo to the submembranous surface of dendritic sites forming excitatory synapses[J]. J Comp Neurol, 2005, 483(4):383-402.
[15] Merriam EB, Lumbard DC, Viesselmann C, et al. Dynamic microtubules promote synaptic NMDA receptor-dependent spine enlargement[J]. PLoS One, 2011, 6(11):e27688. doi:10.1371/journal.pone.0027688.
[16] 马丽娜,王蓉. 大脑发育调节脑蛋白对神经元树突棘及突触可塑性的影响[J]. 中华老年心脑血管病杂志, 2013, 15(10):1109-1111.
[17] Shim KS, Lubec G. Drebrin, a dendritic spine protein, is manifold decreased in brains of patients with Alzheimers disease and Down syndrome[J]. Neurosci Lett, 2002, 324(3):209-212.
[18] Lee D, Aoki C. Presenilin conditional double knockout mice exhibit decreases in drebrin a at hippocampal CA1 synapses[J]. Synapse, 2012, 66(10):870-879.
[19] Volk LJ, Bachman JL, Johnson R, et al. PKM-zeta is not required for hippocampal synaptic plasticity, learning and memory[J]. Nature, 2013, 493(7432):420-423.
[20] Prakash D, Gopinath K, Sudhandiran G. Fisetin enhances behavioral performances and attenuates reactive gliosis and inflammation during aluminum chloride-induced neurotoxicity[J]. Neuromolecular Med, 2013, 15(1):192-208.
[21] Zhao L, Wang JL, Wang YR, et al. Apigenin attenuates copper-mediated beta-amyloid neurotoxicity through antioxidation, mitochondrion protection and MAPK signal inactivation in an AD cell model[J]. Brain Res, 2013, 1492:33- 45.
[1] Jizong ZHAO. Neurosurgery plays a key role in brain science research [J]. Journal of Shandong University (Health Sciences), 2020, 1(8): 1-4.
[2] LI Dandan, LIU Hui, CHENG Aixia. Cloning and functional characterization of an O-methyltransferase gene from Conocephalum conicum [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(12): 20-26.
[3] LI Maoyu, TANG Shi, DU Yifeng. Expression and significance of TRB3 in the brain of APP/PS1 transgenic mice [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(10): 1-5.
[4] L Qing-tao1, ZHONG Xiao-ning2, RONG Rong1, ZHANG Zhao-wang1, SUN Xiu-mei1. HPLC fingerprint-pattern recognition of Yigan granules [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(8): 68-72.
[5] LI Zhe1, YANG Ping2. Cognition level of law of the medical staff in Weihai and its related factors [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(3): 115-119.
[6] LI Zhe1, YANG Ping2. Cognition level of law of the medical staff in Weihai and its related factors [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(3): 115-119.
[7] JIANG Hai-qiang1, LI Yun-lun2, XIE Jun2. Plasma metabonomic study on hypertension by high performance liquid chromatography coupled with time of flight mass spectrometry [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(10): 150-.
[8] WANG De-wei, XIE Zhao-hong, LAI Chao, ZHU Zheng-yu, WANG Yun, JIN Su-qin， BI Jian-zhong. Preliminary application of Montreal cognitive assessment in vascular cognitive impairment [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(9): 97-.
[9] KONG Min， GAO Yuxing. Effect of antiepileptic drugs on cognitive functions and expressions of glutamate receptor 2 and synaptophysin of the hippocampus in rats [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(7): 14-18.
[10] XU Mingming， YI Yonghong. Association between mild cognitive impairment and neprilysin gene rs3736187 polymorphism [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(3): 120-123.
[11] WANG Liyang1, LIAO Tanghong2 , XU Lingzhong2. A survey on the cognition of tuberculosis and its determinants among village doctors [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(2): 157-.
[12] CHEN Li, ZHANG Cheng-qi, WANG Xin-yi, YANG Zhi-qiang. MRI quantitative analysis on cerebellum size in mild cognitive impairment and Alzheimer′s disease patients [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(10): 85-.
[13] . Cognition dysfunction of diabetic rats and its relation to apoptosis in the frontal lobe cortex, the hippocampus and HbA1c [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2009, 47(9): 1-4.
[14] . Role of apolipoprotein E gene polymorphism and plasma lipids in mild cognitive impairment [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2009, 47(11): 103-107.
[15]

SUN Qin-jian,LIU Xue-wu,WANG Ai-hua,ZHANG Tao,CHI Zhao-fu

. Cognitive function in temporal lobe rats and the expression of NR2B in the hippocampus [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2008, 46(7): 676-680.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd