JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (2): 33-37.doi: 10.6040/j.issn.1671-7554.0.2015.705

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Synapse-protective effect of flavonoids extracted from the leaves of Diospyros kaki in APP/PS1 transgenic mice

SHANG Yuying, MA Yingjuan, WU Xiaofan, HOU Xunyao, LUO Dingzhen, CHEN Jian, HONG Yan, SHEN Chao, LIU Xueping   

  1. Department of Senile Neurology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China
  • Received:2015-07-27 Online:2016-02-10 Published:2016-02-10

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Abstract: Objective To investigate the effect of flavonoids extracted from the leaves of Diospyros kaki(FLDK)on cognition levels, synapse structure and the expression of synapse-related proteins in APP/PS1 transgenic mouse. Methods Twenty APP/PS1 mice of four-month old were randomly divided into Alzheimers disease(AD)model group(AD group)and treatment group(AD+FLDK group), ten in each group; ten C57BL/6 mice of four-month old were regarded as normal control group(NC group). AD+FLDK group was treated intragastrically with 80 mg/(kg·d)FLDK, while AD and NC groups were fed intragastrically with the same volume of normal saline. Morris water maze was used to test the escape latency(EL)and number of crossing, transmission electron microscope was used to observe the ultrastructure of synapse, and immunohistochemistry was used to detect the expression levels of synaptophysin and drebrin. Results Compared with NC group, the EL was prolonged, crossing numbers decreased, the synapse ultrastructure was damaged and synapse-related protein levels decreased in AD group. Compared with AD group, EL decreased and crossing number increased obviously, synapse ultrastructure was clearer and synapse-related protein levels increased in AD+FLDK group. Conclusion FLDK can improve cognitive decline in APP/PS1 transgenic mouse, protect the integrity of synapse, and increase the synapse-related protein levels, thus can enhance cognition levels and protect synapse.

Key words: Flavonoids, Cognition, Diospyros kaki, APP/PS1, Synapse

CLC Number: 

  • R741.05
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