超氧化物岐化酶基因多态性与慢性阻塞性肺疾病易感性的关系

山东大学学报(医学版)

超氧化物岐化酶基因多态性与慢性阻塞性肺疾病易感性的关系

韩轶群¹，薛玉文²，李传芬³，车波⁴

1．山东大学医学院，山东济南 250012； 2．山东大学齐鲁医院呼吸内科，山东济南 250012；3．济南军区总医院实验诊断科，山东济南 250031； 4．肥城市人民医院内科，山东肥城 271600

收稿日期:2007-04-17 修回日期:1900-01-01 出版日期:2007-09-24 发布日期:2007-09-24
通讯作者: 薛玉文

The association between genetic polymorphism of superroxide dismutase and chronic obstructive pulmonary diseases

HAN Yi-qun¹，XUE Yu-wen²，LI Chuan-fen³，CHE Bo⁴

1． School of Medicine， Shandong University;2． Department of Respiratory， Qilu Hospital of Shandong University;3． Department of Laboratory Diagnosis， General Hospital of Jinan Military Command;4． Department of Medicine， Feicheng People′s Hospital

Received:2007-04-17 Revised:1900-01-01 Online:2007-09-24 Published:2007-09-24
Contact: XUE Yu-wen

摘要/Abstract

摘要： 目的：探讨超氧化物岐化酶（SOD）基因多态性与慢性阻塞性肺疾病（COPD）易感性的关系。方法：收集70例COPD患者（COPD组）和70例健康人（对照组）空腹外周血，提取全血细胞DNA，采用聚合酶链式反应-限制性片段长度多态性法检测两组SOD1-3基因的多态性。结果：对照组与COPD组SOD1（CuZn-SOD）及SOD2（Mn-SOD）基因多态性无统计学差异，而在SOD3（EC-SOD）基因多态性方面有统计学差异（P＜0.05）。结论：SOD3（EC-SOD）基因多态性（C+760G，Arg213Gly）与COPD易感性有关，可能是COPD的易感基因。

Abstract: Objective： To explore the association of genetic polymorphism of superoxide dismutase（SOD） and chronic obstructive pulmonary diseases（COPD）. Methods： 70 patients with COPD （the COPD group）and 70 healthy volunteers (the control group ) were enrolled in this study. Limosis peripheral blood was taken and whole blood corpuscle genomic DNA was extracted. The genetic polymorphism of SOD1-3 genes was determined by polymerase chain reaction and restriction fragment length polymorphism(PCR-RFLP). Result： There were no logistic differences between the COPD and the control groups in the genetic polymorphism of SOD1（CuZn-SOD） and SOD2（Mn-SOD）, however, there were logistic differences in the genetic polymorphism of SOD3（EC-SOD）（P＜0.05）. Conclusion： SOD3（EC-SOD）genetic polymorphism (C+760G，Arg213Gly) is related to the development of COPD and it may be the COPDrelated gene.

Key words: Superoxide dismutase, Genetic polymorphism, Chronic obstructive pulmonary disease, Polymerase chain reaction, Polymorphism, restriction fragment length

中图分类号:

R392.21

韩轶群,薛玉文,李传芬,车波 . 超氧化物岐化酶基因多态性与慢性阻塞性肺疾病易感性的关系[J]. 山东大学学报(医学版), 2007, 45(9): 940-943.

HAN Yi-qun,XUE Yu-wen,LI Chuan-fen,CHE Bo. The association between genetic polymorphism of superroxide dismutase and chronic obstructive pulmonary diseases[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2007, 45(9): 940-943.

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