GM-1对大鼠视网膜缺血再灌注损伤后NF-κB表达的影响

山东大学学报(医学版)

GM-1对大鼠视网膜缺血再灌注损伤后NF-κB表达的影响

肖迎¹，王琪¹，张效房²，金学民²，张金嵩²

1．山东省立医院眼科，山东济南 250021；2．郑州大学附属第一医院眼科，河南郑州 450052

收稿日期:2007-04-14 修回日期:1900-01-01 出版日期:2007-09-24 发布日期:2007-09-24
通讯作者: 张效房

Effect of GM-1 on NF-κB in rat retinas after ischemia-reperfusion injury

XIAO Ying¹，WANG Qi¹，ZHANG Xiao-fang²，JIN Xue-min²，ZHANG Xue-song²

1.Department of Ophthalmology, Shandong Provincial Hospital；2.Department of Ophthalmology, First Affiliated Hospital of Zhengzhou University

Received:2007-04-14 Revised:1900-01-01 Online:2007-09-24 Published:2007-09-24
Contact: ZHANG Xiao-fang

摘要/Abstract

摘要： 目的：观察神经节苷脂(monosialoganglioside，GM-1)对大鼠视网膜缺血再灌注损伤后视网膜结构的保护作用及对核因子-κB(nuclear factor-kappaB, NF-κB )表达的影响，探讨其可能的保护机制。方法：健康成年Wistar大鼠75只，随机分为3组：正常组5只、NS组35只、GM-1组35只。正常组不加任何处理因素，NS组和GM-1组通过升高眼压造成视网膜缺血60min，分别于缺血前12、1h及缺血结束时3次腹腔注射NS 3ml·kg-1或GM-1 3ml·kg-1，并于再灌注0(单纯缺血后)、1、6、12、24、72和168h共7个时点取双眼眼球，每时间点5只。HE染色观察视网膜组织结构并测量视网膜内层平均厚度(mean thickness of the inner retinal layers, MTIRL)，免疫组化SP法检测NF-κB的表达并计算阳性细胞率。方差分析法进行统计处理。结果：大鼠视网膜缺血再灌注后，内层视网膜相继出现水肿和萎缩。再灌注后大鼠视网膜内层NF-κB迅速激活，随后其表达逐渐下降。GM-1可明显减轻视网膜缺血再灌注后的组织损伤，并显著抑制NF-κB的活化。结论：NF-κB活化可能是视网膜缺血再灌注损伤的早期始动因素。GM-1对视网膜缺血再灌注损伤具有明确的保护作用，对NF-κB的抑制可能是其保护机制之一。

Abstract: Objective: To investigate the protective effect of monosialoganglioside GM-1 on rat retinal tissues and the influence of GM-1 on NF-κB after ischemia-reperfusion injury. Methods: Seventy-five healthy adult Wistar rats were randomly divided into 3 groups: the control group(n=5), the NS group(n=35) and the GM-1 group(n=35). The control group received no treatment. Rats in the other 2 groups received complete retinal ischemia by increasing the intraocular pressure for 60 minutes and they were intraperitonially injected to 3 ml·kg-1 NS or 3 ml·kg-1 GM-1 at 12 and 1 hour before and immediately after ischemia. According to the survival time after reperfusion, the experimental 2 groups were subdivided into 7 time points: 0 (only ischemia), 1, 6, 12, 24, 72 and 168 hours after ischemia. Morphological changes and mean thickness of the inner retinal layers (MTIRL) of the retina were determined under light microscopy. Expression of NFκB was determined by the immunohistochemistry method. Results: Rats inner retinas had the lesion process of edema and atrophy after ischemiareperfusion injury.gradually decreased. Pathological changes of retina and activation of NFκB were obviously relieved by GM-1. Conclusion: GM-1 definitely protects against retinal ischemia-reperfusion injury and it is a potential agent for retinal ischemia-reperfusion injury.

Key words: Ganglioside, Retina, Reperfusion injury, NF-κB

中图分类号:

R774.1

肖迎,王琪,张效房,金学民,张金嵩 . GM-1对大鼠视网膜缺血再灌注损伤后NF-κB表达的影响[J]. 山东大学学报(医学版), 2007, 45(9): 910-913.

XIAO Ying,WANG Qi,ZHANG Xiao-fang,JIN Xue-min,ZHANG Xue-song. Effect of GM-1 on NF-κB in rat retinas after ischemia-reperfusion injury[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2007, 45(9): 910-913.

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