基于RNA高通量测序研究右美托咪定对人神经母细胞瘤细胞基因表达的影响

doi:10.6040/j.issn.1671-7554.0.2022.1434

摘要/Abstract

摘要： 目的分析右美托咪定对人神经母细胞瘤SH-SY5Y细胞表达谱差异的影响,探讨右美托咪定在脑保护中的潜在机制。方法培养SH-SY5Y细胞,分为实验组(T组,生物学重复n=3)和对照组(C组,生物学重复n=3)。实验组用右美托咪定刺激48 h,对照组相同条件下用DMSO处理。使用Trizol提取RNA,在Illumina Novaseq平台进行RNA测序。采用Limma分析实验组与对照组的差异基因,并对差异基因进行基因本体(GO)富集分析及京都基因与基因组百科全书(KEGG)信号通路富集分析。结果 T组与C组相比,共有211个差异表达基因(上调105个,下调106个)。其中FGF1、GNG13、P2RY4在实验组和对照组中表达差异明显,差异倍数分别为2.23、2.00和6.56。GO富集分析示差异基因主要富集于磷脂酶C活化的G蛋白偶联受体信号通路、蛋白激酶B信号的调控、BMP信号通路。KEGG通路富集分析示差异基因主要富集于白细胞跨内皮迁移。结论 FGF1、GNG13、P2RY4等基因可能参与右美托咪定对脑的保护作用。右美托咪定可能主要通过炎症机制参与对中枢神经系统的保护。

关键词: 右美托咪定, RNA高通量测序, SH-SY5Y细胞, 脑保护, 中枢神经系统

Abstract: Objective To investigate the potential mechanism of Dexmedetomidine in brain protection by analyzing its effects on the gene expression of SH-SY5Y cells. Methods SH-SY5Y cells were cultured and divided into the experimental group(T group, biological replicate n=3)and control group(C group, biological replicate n=3). The experimental group was stimulated with Dexmedetomidine for 48 hours, while the control group was treated with DMSO under the same conditions. RNA was extracted with Trizol reagent and sequenced with Illumina Novaseq. The differentially expressed genes between the two groups were identified with Limma, and analyzed with gene ontology(GO)enrichment analysis and Kyoto encyclopedia of genes and genomes(KEGG)pathway enrichment analysis. Results A total of 211 differentially expressed genes were identified, including 105 up-regulated and 106 down-regulated ones. The expressions of FGF1, GNG13 and P2RY4 were significantly different between the two groups, and the difference folds were 2.23, 2.00 and 6.56, respectively. GO enrichment analysis showed that the differentially expressed genes were mainly enriched in phospholipase C-activating G protein-coupled receptor signaling pathway, regulation of protein kinase B signaling and BMP signaling pathway, while KEGG pathway enrichment analysis revealed that they were mainly enriched in leukocyte transendothelial migration. Conclusion Genes including FGF1, GNG13 and P2RY4 may be involved in the protective effects of Dexmedetomidine on brain. Dexmedetomidine protects the central nervous system through inflammatory mechanism.

Key words: Dexmedetomidine, RNA high-throughput sequencing, SH-SY5Y cells, Brain protection, Central nervous system

中图分类号:

R614

管伟,白云峰,杜胜杰,王月兰,李希明. 基于RNA高通量测序研究右美托咪定对人神经母细胞瘤细胞基因表达的影响[J]. 山东大学学报 (医学版), 2023, 61(7): 27-33.

GUAN Wei, BAI Yunfeng, DU Shengjie, WANG Yuelan, LI Ximing. Effects of Dexmedetomidine on the gene expressions of SH-SY5Y cells by RNA high-throughput sequencing[J]. Journal of Shandong University (Health Sciences), 2023, 61(7): 27-33.

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