液体-固体联合培养法检测泌尿生殖道支原体的临床研究

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doi:10.6040/j.issn.1671-7554.0.2018.456

摘要： 目的通过对比液体培养法、固体培养法、液体-固体联合培养法的支原体检测结果,探讨支原体检测的优化方法。方法收集2016年10月至2017年8月宫颈分泌物标本1 642份,采用液体培养法、固体培养法和液体-固体联合培养法平行检测支原体,于24、48 h分别判读解脲支原体(Uu)和人型支原体(Mh)液体培养结果,48 h判读固体培养结果,根据检测结果计算阳性率和假阳性率,用χ²检验分析数据。结果液体培养法总阳性率为40.38%,其中Uu、Mh及混合感染阳性率分别为 30.09%、5.42%、4.87%,假阳性率为5.67%;固体培养法总阳性率为39.46%,其中Uu、Mh及混合感染阳性率分别为32.22%、2.80%、4.44%,假阳性率为0.37%;液体-固体联合培养法的阳性率为32.70%,其中Uu、Mh及混合感染阳性率分别为25.70%、2.74%、4.26%,假阳性率为0.12%。液体-固体联合培养法与固体培养法/液体培养法在支原体阳性率上差异有统计学意义(P<0.05),液体培养法与固体培养法/液体-固体联合培养法在假阳性率上差异有统计学意义(P<0.05)。结论液体-固体联合培养法能够准确培养鉴定Uu和Mh,既能排除女性生殖道支原体的正常定植,同时又能够显著降低由于细菌或真菌污染所导致液体培养法产生假阳性结果的情况。

关键词: 支原体, 液体培养法, 固体培养法, 液体-固体联合培养法

Abstract: Objective To compare the liquid medium culture, solid medium culture and liquid-solid medium culture of mycoplasma detection methods, and to optimize culture methods for mycoplasma. Methods A total of 1 642 samples from female genital tract were cultured by the liquid medium culture, solid medium culture and liquid-solid medium culture parallelly from October 2016 to August 2017. The liquid culture results of ureaplasma urealyticum(Uu)and mycoplasma hominis(Mh)were interpretated at 24 and 48 h, respectively; the solid culture result was interpretated at 48 h. The positive rate and false positive rate were calculated and statistical data were analyzed by χ² test. Results The total positive rate of liquid culture method was 40.38%, among which the positive rates of Uu, Mh and mixed infection were 30.09%, 5.42%, and 4.87%, respectively, and the false positive rate was 5.67%; the total positive rate of solid culture method was 39.46%, among which the positive rates of Uu, Mh and mixed infection were 32.2%, 2.80%, and 4.44%, respectively, and the false positive rate was 0.37%; the total positive rate of the liquid-solid culture method was 32.70%, among which the positive rates of Uu, Mh and mixed infection were 25.70%, 2.74%, and 4.26%, respectively, and the false positive rate was 0.12%. The positive rate exhibited a statistical difference between the liquid-solid medium culture method and liquid medium culture method/solid medium culture method(χ²=16.267, P<0.001; χ²=20.848, 山东大学学报 (医学版)56卷10期 -贾忠兰,等.液体-固体联合培养法检测泌尿生殖道支原体的临床研究 \=-P<0.001). The false positive rate exhibited a statistical difference between the liquid medium culture method and solid medium culture method(χ²=5.192, P=0.019)/ liquid-solid medium culture method(χ²=4.851, P=0.033). Conclusion The combination of liquid culture and solid culture can accurately identify Uu and Mh. It can eliminate mycoplasma colonizationn in female genital tract and significantly reduce the false positive result caused by the liquid culture method due to bacterial or fungal contamination.

Key words: Mycoplasma, Liquid medium culture method, Solid medium culture method, Liquid-solid medium culture method

中图分类号:

R375

贾忠兰,许丽风. 液体-固体联合培养法检测泌尿生殖道支原体的临床研究[J]. 山东大学学报 (医学版), 2018, 56(10): 110-113.

JIA Zhonglan, XU Lifeng. Clinical research on liquid-solid medium culture method in the detection of genitourinary tract mycoplasma infection[J]. Journal of Shandong University (Health Sciences), 2018, 56(10): 110-113.

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