山东大学学报（医学版） ›› 2017, Vol. 55 ›› Issue (9): 41-45.doi: 10.6040/j.issn.1671-7554.0.2016.1656
秦鲁敏,孙爱友,王倩文,高景文,魏东芝
QIN Lumin, SUN Aiyou, WANG Qianwen, GAO Jingwen, WEI Dongzhi
摘要： 目的 建立一种高残糖-pH反复重碱化震荡发酵(F4)策略,提高乳酸片球菌素(pediocin)的发酵效价。 方法 在发酵的第一阶段采用间歇发酵,初始葡萄糖浓度为8.8 g/L;待发酵液中的残留葡萄糖浓度低于2 g/L时开始第二阶段发酵;在第二阶段,使发酵液中的残糖浓度始终维持在20 g/L左右,同时,每隔6 h将发酵液的pH值调到6.5,维持pH值重碱化震荡。 结果 由F4策略发酵产生的pediocin单位效价是对照策略F1和F2的3倍、F3的1.7倍,达到24 000 AU/mL。 结论 F4策略既能维持菌体的稳定生长,又可大量积累pediocin,适用于大规模生产pediocin的补料流加。
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