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山东大学学报（医学版） ›› 2017, Vol. 55 ›› Issue (7): 31-37.doi: 10.6040/j.issn.1671-7554.0.2017.198

• 基础医学 • 上一篇    下一篇

不同浓度一氧化碳释放分子-3对大鼠骨髓间充质干细胞成骨分化的影响

李景媛1,宋玲2,林松3,宋晖4   

  1. 1. 山东大学口腔医院黏膜病科, 山东省口腔组织再生重点实验室, 山东 济南 250012;2. 青岛市市立医院口腔科, 山东 青岛 266011;3. 济南市口腔医院儿童牙病科, 山东 济南 250001;4. 山东大学口腔医院特诊科, 山东省口腔组织再生重点实验室, 山东 济南 250012
  • 收稿日期:2017-03-07 出版日期:2017-07-10 发布日期:2017-07-10
  • 通讯作者: 宋晖. E-mail:songhui@sdu.edu.cn林松. E-mail:ls196868@163.com E-mail:songhui@sdu.edu.cn
  • 基金资助:
    山东省自然科学基金(ZR2015HM019);山东省教育奖补专项基金(鲁财教指［2014］94号);济南市高校科技创新计划项目(201401259)

Effects of carbon monoxide releasing molecule-3 on the osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells

LI Jingyuan1, SONG Ling2, LIN Song3, SONG Hui4   

  1. 1. Department of Oral Mucosal Diseases, Stomatological Hospital of Shandong University, Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Jinan 250012, Shandong, China;
    2. Department of Stomatology, Qingdao Municipal Hospital, Qingdao 266011, Shandong, China;
    3. Department of Pediatric Dentistry, Jinan Stomatological Hospital, Jinan 250001, Shandong, China;
    4. Department of VIP Clinic, Stomatological Hospital of Shandong University, Shandong Provincial Key Laboratory ofOral Tissue Regeneration, Jinan 250012, Shandong, China
  • Received:2017-03-07 Online:2017-07-10 Published:2017-07-10

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摘要： 目的 探讨不同浓度一氧化碳释放分子-3(CORM-3)对大鼠骨髓间充质干细胞(BMSCs)成骨分化的影响。 方法 实验分A、B两部分,各部分均包括5组,A部分:矿化组,100、200、400 μmol/L CORM-3组,对照组;B部分:矿化组,100、200、400 μmol/L CORM-3-矿化组,对照组。采用Real-time PCR法和Western blotting法分别检测成骨相关基因Runx2和OPNmRNA与蛋白的表达,茜素红染色检测成骨能力。采用SPSS 19.0软件对数据进行统计学分析。 结果 100、200 μmol/L CORM-3可促进BMSCs增殖,差异有统计学意义(P=0.034,P<0.001);单纯CORM-3对BMSCs成骨分化无影响;BMSCs经200、400 μmol/L CORM-3预处理后再行矿化诱导,7 d后大鼠BMSCs的Runx2和OPNmRNA表达增强,与矿化组比较差异有统计学意义(Runx2:P=0.005,P=0.006;OPN:P=0.010,P=0.028),其蛋白表达及21 d后的成骨能力较矿化组均明显增强,且200 μmol/L CORM-3预处理的效果优于400 μmol/L CORM-3。 结论 CORM-3预处理可促进体外BMSCs成骨分化,200 μmol/L为CORM-3用于实验的适宜浓度。

关键词: 一氧化碳释放分子-3, 骨髓间充质干细胞, Runt相关转录因子2, 骨桥蛋白, 成骨分化

Abstract: Objective To investigate the effects of carbon monoxide releasing molecule-3(CORM-3)on the osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells(BMSCs). Methods The BMSCs were divided into 2 groups, group A and group B. Group A was subdivided into osteogenic group, 100 μmol/L CORM-3 group, 200 μmol/L CORM-3 group, 400 μmol/L CORM-3 group and control group. Group B was subdivided into osteogenic 山 东 大 学 学 报 (医 学 版)55卷7期 -李景媛,等.不同浓度一氧化碳释放分子-3对大鼠骨髓间充质干细胞成骨分化的影响 \=-group, 100 μmol/L CORM-3-osteogenic group, 200 μmol/L CORM-3-osteogenic group, 400 μmol/L CORM-3-osteogenic group and control group. The mRNA and protein expressions of Runx2 and OPN were detected with Real-time PCR and Western blotting. The cell mineralization was determined with alizarin red staining. Data obtained were analyzed with SPSS 19.0 software. Results CORM-3 at concentrations of 100 μmol/L and 200 μmol/L could significantly promote the proliferation of BMSCs(P=0.034, P<0.001). The mRNA expressions of Runx2 and OPN in 200 μmol/L and 400 μmol/L CORM-3-osteogenic groups were significantly higher than those in the osteogenic group on day 7(Runx2: P=0.005, P=0.006; OPN: P=0.010, P=0.028). Meanwhile, the protein expressions of Runx2 and OPN on day 7 and cell mineralization on day 21 in 200 μmol/L and 400 μmol/L CORM-3-osteogenic groups were enhanced compared with the osteogenic group, and the 200 μmol/L group had better results than the 400 μmol/L group. Conclusion CORM-3 may promote osteogenic differentiation of BMSCs and 200 μmol/L is the suitable concentration.

Key words: Carbon monoxide releasing molecules-3, Osteogenic differentiation, Bone marrow-derived mesenchymal stem cells, Runt-related transcription factor 2, Osteopontin

中图分类号: 

  • R781.4
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