枯草芽孢杆菌灌胃对小鼠肠道菌群的影响

doi:10.6040/j.issn.1671-7554.0.2017.076

摘要/Abstract

摘要： 目的探讨枯草芽孢杆菌(Bacillus subtilis)服用时间长短对小鼠肠道菌群的影响。方法 C57BL/6J雌鼠18只随机分为3组(n=6):对照组(C组)从第0天至第9天每天以生理盐水灌胃;2 d处理组(TW组)从第0天至第1天每天以B.subtilis活菌灌胃,第2天至第9天继续以等量生理盐水灌胃;10 d处理组(TE组)于第0天至第9天以等量的B.subtilis活菌灌胃。小鼠从第0天至第16天每天自由饮食。在第0天、第8天和第16天收取所有小鼠的粪便,提取DNA后通过Illumina Miseq高通量测序平台测序。结果灌胃后,TW组和TE组的芽孢杆菌属微生物相对丰度与灌胃前相比下降,且TE组差异具有统计学意义(P=0.039)。各组内肠道菌群相对丰度随时间变化趋势为:TW组和TE组中厚壁菌门相对丰度先上升后下降,变形菌门相对丰度则呈相反情况;疣微菌门相对丰度逐步上升,TW组第16天、第8天与灌胃前相比差异有统计学意义(P=0.001),且在第16天时TW组和TE组与C组比较其相对丰度差异有统计学意义(P=0.007)。TE组第8天与第16天的小鼠肠道菌群结构相似,而TW组在这两个时间点的肠道菌群结构有差异。第16天时,TW组和TE组小鼠肠道菌群结构相似。结论枯草芽孢杆菌并非通过定植,而是通过与肠道菌群相互作用使其结构发生改变的方式发挥益生功效;长期服用该益生菌对肠道菌群结构的改变速度快于短期服用。

关键词: 肠道菌群, 益生菌, 高通量测序, 枯草芽孢杆菌

Abstract: Objective To investigate the effect of Bacillus subtilis B.subtilis gavage on the intestinal microbiota of mice. Methods Eighteen C57BL/6J female mice were randomly divided into three groups including control group(C group), two days intervention group(TW group)and ten days intervention group(TE group). C group was treated with normal saline each day from day 0 to day 9; TW group was treated with live B.subtilis each day from day 0 to day 1, then with normal saline at the same amount from day 2 to day 9; TE group was treated with live B.subtilis at the same amount each day from day 0 to day 9. All mice were given an unrestricted diet from day 0 to day 16.Feces were collected on day 0, day 8 and day 16 to extract DNA, then Illumina Miseq platform was used for high-throughput sequencing. Results After treated with live B.subtilis, the relative abundance of Bacillus genus microbes in TW group and TE group decreased, and in the TE group the difference was significantly different(P=0.039). The relative abundance of Firmicutes increased first then decreased in both TW group and TE group, while Bacteroidetes shows reverse trend. The relative abundance of Verrucomicrobia increased gradually, which in TW group showed significant difference on the sixteenth day and eighth day compared with that before gavage(P=0.001). The relative abundances of Verrucomicrobia in TW group, TE group and C group on the sixteenth day were significantly different(P=0.007). Meanwhile, it was found that the intestinal flora structure of mice on the eighth day was similar to that on the sixteenth day in TE group, while the intestinal flora structure of mice in TW group differed siginificantly. On the sixteenth day, the intestinal flora structure of TW group was similar to that of TE group. Conclusion B.subtilis could regulate intestinal flora structure possibly via interacting with intestinal microbia but not via colonizing. Long-term use of the probiotic 山东大学学报 (医学版)55卷10期 -赵力,等.枯草芽孢杆菌灌胃对小鼠肠道菌群的影响 \=-changes intestinal flora structure more quickly than short-term does.

Key words: High-throughput sequencing, Probiotics, Intestinal microbiota, Bacillus subtilis

中图分类号:

R574

赵力,李理想,陈飞雪,左秀丽,李延青. 枯草芽孢杆菌灌胃对小鼠肠道菌群的影响[J]. 山东大学学报（医学版）, 2017, 55(10): 28-35.

ZHAO Li, LI Lixiang, CHEN Feixue, ZUO Xiuli, LI Yanqing. Effect of Bacillus subtilis gavage on the intestinal microbiota of mice[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(10): 28-35.

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